A) Cysts in a vessel wall (arrows) of the patient (hematoxylin and eosin stain, magnification ×250). Inset shows a cyst at higher magnification (hematoxylin and eosin stain, magnification ×800). B) Immunohistochemical staining with antibody to en:Acanthamoeba cysts within vessel walls (magnification ×250). C) Polyacrylamide gel electrophoresis of PCR products for Acanthamoeba spp., en:Naegleria spp., and en:Balamuthia mandrillaris using JDP primers for a diagnostic small subunit rDNA fragment. M, molecular mass marker; Pat, patient; Neg, negative control; Pos, positive control.

Source:Meersseman W, Lagrou K, Sciot R, de Jonckheere J, Haberler C, Walochnik J, Peetermans W, van Wijngaerden E. "Rapidly Fatal Acanthamoeba Encephalitis and Treatment of Cryoglobulinemia". Emerg Infect Dis [serial on the Internet]. 2007 March [cited 2007 Feb 23]. Available from https://www.cdc.gov/eid/content/13/3/469-G2.htm