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مستخدم:Noor khderat/ملعب

من ويكيبيديا، الموسوعة الحرة

spectrophotometer

It is a device that measures the extent to which a particular chemical absorbs light when a beam of light passes through its solution. The idea of the principle of the device is simple as each of .

the chemicals absorbs light better at some wavelengths than other materials, and this device can also be used to measure The amount and concentration of a chemical is known


Spectrophotometric techniques are mostly used to measure the concentration of solutes in the solution by exposing the sample to a certain amount of light and measuring the amount of light absorbed by the solution placed in the device. The higher the concentration of this substance, the greater the amount of absorbed light. To calculate the concentration, the calculated readings must be compared with the readings of known concentration standards



SpectroPhotometer Applications

Qualitative Analysis

By using the visible spectrum and ultraviolet rays to know the class and type of the compound, by drawing the absorption spectrum curves, where the compound’s absorption of light in different areas gives indications of the quality or nature of the material.


Enzyme Assay

It is the main application of the spectrophotometer. This test is performed quickly and easily when the chemical absorbs light in the ultraviolet range.



These are from the basic spectrophotometer instrumentation and its applications



https://chem.libretexts.org/Bookshelves/Physical_and_Theoretical_Chemistry_Textbook_Maps/Supplemental_Modules_%28Physical_and_Theoretical_Chemistry%29/Kinetics/02%3A_Reaction_Rates/2.01%3A_Experimental_Determination_of_Kinetics/2.1.05%3A_Spectrophotometry


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